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Home Antibody All anti-ITGB2 antibodies

Anti-ITGB2 Antibody EP1288Y

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA300815 Rabbit Monoclonal Antibody against ITGB2 (Clone EP1288Y) 100ul $325 In Stock
LC400076 ITGB2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(1)
IHC(1)
IF(1)
FC(1)

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Ser745 of human LFA-?2 was used as immunogen. The antibody only detects LFA-?2 phosphorylated on Serine 745.
Clone NameEP1288Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:500; IP: 1:100; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:250 - 1:500; FC: 1:1000
Predicted MW Explanation 85 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) (ITGB2), transcript variant 1
Alternative NameCD18; LAD; LCAMB; LFA-1; MAC-1; MF17; MFI7
Database LinkNP_000202
Entrez Gene 3689 Human
Entrez Gene 16414 Mouse
Entrez Gene 309684 Rat
FunctionIn the immune system, integrins have essential roles in leukocyte trafficking and function. These include immune cell attachment to endothelial and antigen-presenting cells, cytotoxicity, and extravasation into tissues (1). Integrin adhesion receptors transduce signals that control complex cell functions which require the regulation of gene expression, such as proliferation, differentiation and survival. Their intracellular domain has no catalytic function, indicating that interaction with other transducing molecules is crucial for integrin-mediated signaling. JAB1 (Jun activation domain-binding protein 1), a coactivator of the c-Jun transcription factor, has been identified as a protein that interacts with the cytoplasmic domain of the beta2 subunit of the alphaL/beta2 integrin LFA-1 (2). Patients with leukocyte adhesion molecule (CD11/CD18, beta 2 integrins) deficiency have structural defects in the common beta subunit (CD18), which prevent heterodimer formation and normal cell surface expression of these receptors, leading to life-threatening bacterial infections (3)
Related PathwayTransmembraneDruggable Genome Cell adhesion molecules (CAMs)Natural killer cell mediated cytotoxicityLeukocyte transendothelial migrationRegulation of actin cytoskeletonViral myocarditis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - CD18 (phospho S745) antibody [EP1288Y]; All lanes : Anti-CD18 (phospho S745) antibody [EP1288Y] at 1/500 dilution.Lane 1 : Jurkat cell lysate, untreated.Lane 2 : Jurkat cell lysate, treated with AP.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 85 kDa.Observed band size : 85 kDa.
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Immunohistochemistry (Paraffin-embedded sections) - CD18 (phospho S745) antibody [EP1288Y]; Ab52920 (1:250) staining human CD18 in human tonsil tissue by immunohistochemistry using paraffin embedded tissue.
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Immunocytochemistry/ Immunofluorescence - Anti-CD18 (phospho S745) antibody [EP1288Y]; ICC/IF image of TA300815 stained Raw 246.7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody TA300815 at 1/1000 dilution overnight at +4°C. The secondary antibody (green) was DyLight? 488 goat anti- rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
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Flow Cytometry - Anti-CD18 (phospho S745) antibody [EP1288Y]; Human peripheral blood lymphocytes stained with TA300815 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody for 30 min at 4°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1??/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy ??peripheral blood lymphocytes.
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