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Anti-ITGB1 Antibody EP1041Y
Also for ITGB1 (NM_002211)
|A synthetic peptide corresponding to the residue near the C-terminal of human Integrin beta-1 (CD29) was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||ICC/IF: Use at an assay dependent dilution; WB: 1:500; FC: 1:70 - 1:100; IHC-Fr: Use at an assay dependent dilution; IHC-P: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12) (ITGB1), transcript variant 1A|
|CD29; FNRB; GPIIA; MDF2; MSK12; VLA-BETA; VLAB|
|Integrin beta-1 (ITGB1, CD29, VLA-beta) is the beta subunit found in the integrin families, forming a heterodimer integrin receptor through non-covalent bonding with various integrin alpha subunits. Integrin heterodimer containing Integrin beta-1 binds to various cell surface and extracellular proteins (CD49a-f, CD51) to mediate cell to cell and cell to matrix adhesion (1). Integrin beta-1 plays a critical role in the cell adhesion and recognition in embryogenesis, hemostasis, immune response, tissue repair, metastatic diffusion of tumor cells and development (2, 3, 4).|
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Western blot - Integrin beta 1 antibody [EP1041Y]; Anti-Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end at 1/500 dilution + U937 cell lysate at 10 µg.Secondary.goat anti-rabbit HRP labelled at 1/2000 dilution.Predicted band size : 88 kDa.Observed band size : 140 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Integrin beta 1 antibody [EP1041Y]; ab52971, at a 1/250 dilution, staining Human Integrin beta 1 in breast carcinoma using Immunohistochemistry, Paraffin Embedded tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end; ab52971 staining human breast cancer metastasis tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking with a commercial blocking reagent and incubation with the antibody (diluted 1/100) for 18 hours at 4°C. A HRP-conjugated goat anti-rabbit was used as the secondary antibody. This image shows a cancer metastasis at 40x with beta1 staining (in red) in both blood vessels and tumour cells. Blue is Hoechst for nuclei. The antibody detection was enhanced using a commercial Cy3 tyramide signal amplification kit.
Immunocytochemistry/ Immunofluorescence - Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end; ICC/IF image of ab52971 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end; ab52971 at a 1/400 dilution staining Integrin beta 1 in rat medullary thyroid carcinoma cells cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed with 4% paraformaldehyde for 15 minutes, blocked for 30 minutes using 5% goat serum (nonpermeabilized). The secondary antibody used was a DyLight 549-conjugated goat anti-rabbit at a 1/500 dilution.
Flow Cytometry - Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end; Overlay histogram showing MCF-7 cells stained with ab52971 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.