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Home Antibody All anti-ISG15 antibodies

Anti-ISG15 Antibody EPR3445

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307250
  • Rabbit monoclonal antibody against ISG15 (clone EPR3445 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417516) , 20ug
100ul 325 In Stock
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WB(1)
IHC(1)
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Also for ISG15 (NM_005101)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human ISG15 was used as an immunogen.
Clone NameEPR3445 IsotypeIgG
Species ReactivityHuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsICC: 1:100 - 250, WB: 1:2000 - 5000,
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens ISG15 ubiquitin-like modifier (ISG15)
Alternative NameG1P2; hUCRP; IFI15; IP17; UCRP
Database LinkNP_005092
FunctionISG15 is a ubiquitin like protein that is covalently conjugated to cellular proteins after IFN-alpha/beta stimulation (2). ISG15 is conjugated to their targets by an enzymatic cascade involving an E1 activating enzyme, an E2 conjugating enzyme, and in some cases an E3 ligase. ISG15 conjugation plays an important role by impacting nuclear and cytoplasmic functions as well as in antiviral response because a human virus, influenza B virus, inhibits ISG15 conjugation. However, the biological function of ISG15 modification remains unknown, largely because only a few human ISG15 target proteins have been identified (3).
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - ISG15 antibody [EPR3445]; All lanes : Anti-ISG15 antibody [EPR3445] at 1/2000 dilution.Lane 1 : Hela cell lysate treated with IFN α.Lane 2 : Daudi cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 15 kDa.Observed band size : 15 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISG15 antibody [EPR3445]; Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, staining ISG15 with TA307250 at 1/100 dilution.

 

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