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Anti-ISG15 Antibody EPR3445
Also for ISG15 (NM_005101)
|A synthetic peptide corresponding to residues in human ISG15 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||ICC: 1:100 - 250, WB: 1:2000 - 5000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens ISG15 ubiquitin-like modifier (ISG15)|
|G1P2; hUCRP; IFI15; IP17; UCRP|
|ISG15 is a ubiquitin like protein that is covalently conjugated to cellular proteins after IFN-alpha/beta stimulation (2). ISG15 is conjugated to their targets by an enzymatic cascade involving an E1 activating enzyme, an E2 conjugating enzyme, and in some cases an E3 ligase. ISG15 conjugation plays an important role by impacting nuclear and cytoplasmic functions as well as in antiviral response because a human virus, influenza B virus, inhibits ISG15 conjugation. However, the biological function of ISG15 modification remains unknown, largely because only a few human ISG15 target proteins have been identified (3). |
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Western blot - ISG15 antibody [EPR3445]; All lanes : Anti-ISG15 antibody [EPR3445] at 1/2000 dilution.Lane 1 : Hela cell lysate treated with IFN α.Lane 2 : Daudi cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 15 kDa.Observed band size : 15 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISG15 antibody [EPR3445]; Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, staining ISG15 with ab92345 at 1/100 dilution.