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Anti-IRS1 PHOSPHO Antibody
Also for IRS1 (NM_005544)
|The antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 636 (P-M-SP-P-K).|
|Human, Mouse, Rat
|WB, IHC, IF
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:20000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|IRS-1 (Phospho-Ser636) antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 636.
|Homo sapiens insulin receptor substrate 1 (IRS1)|
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Western blot analysis of extracts from 293 cells and COLO205 cells, using IRS-1 (Phospho-Ser636) antibody.The lane on the right is treated with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using IRS-1 (Phospho-Ser636) antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of MCF7 cells, using IRS-1 (Phospho-Ser636) antibody.The picture on the right is treated with the synthesized peptide.