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Also for IRS1 (NM_005544)
|The antiserum was produced against synthesized non-phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 312 (A-T-SP-P-A).|
|Human, Mouse, Rat
|WB, IHC, IF
||WB: 1:500~1:3000, IHC: 1:50~1:100, IF: 1:100~1:500, ELISA: 1:20000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|IRS-1 (Ab-312) antibody detects endogenous levels of total IRS-1 protein.
|Homo sapiens insulin receptor substrate 1 (IRS1)|
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Western blot analysis of extracts from NIH-3T3 cells, using IRS-1 (Ab-312) Antibody.The lane on the right is treated with the synthesized peptide.Western blot analysis of extracts from JK cells (Lane 2), using IRS-1 (Ab-312) Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using IRS-1 (Ab-312) Antibody.The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using IRS-1 (Ab-312) Antibody.The picture on the right is treated with the synthesized peptide.