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Anti-IRF4 Antibody EPR5653
Also for IRF4 (NM_002460)
|A synthetic peptide corresponding to residues on the N-terminus in human IRF-4 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IHC
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt or ICC.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or ICC.
|Homo sapiens interferon regulatory factor 4 (IRF4), transcript variant 1|
|LSIRF; MUM1; NF-EM5|
|IRF-4 belongs to the IRF (interferon regulatory factor) family of transcription factors, characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by viruses and the regulation of interferon-inducible proteins.IRF-4 is lymphocyte specific and negatively regulates Toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A mutation in IRF-4 may be a cause of multiple myeloma (1).|
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Western blot - Anti-MUM1 antibody [EPR5653]; All lanes : Anti-MUM1 antibody [EPR5653] at 1/1000 dilution.Lane 1 : Ramos cell lysate.Lane 2 : HuT 78 cell lysate.Lane 3 : IM 9 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 52 kDa.
Other-Anti-MUM1 antibody [EPR5653](ab124691); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [EPR5653]; ab124691, at 1/100, staining MUM1 in paraffin-embedded Human colon tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [EPR5653]; ab124691, at 1/100, staining MUM1 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [EPR5653]; Immunohistochemical analysis of dog spleen tissue, staining MUM1 with ab124691.Tissue was fixed with formaldehyde and blocked with 2.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a TRIS-EDTA buffer (pH 9). Samples were incubated with primary antibody (1/50 in PBS plus 1X casein) for 1 hour 30 minutes at 37°C. An undiluted HRP-conjugated horse anti-rabbit polyclonal IgG was used as the secondary antibody.