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Home Antibody All anti-IRF4 antibodies

Anti-IRF4 Antibody EPR5653

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310950
  • Rabbit Monoclonal antibody against IRF-4 (IRF4)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC419304) , 20ug
100ul $325 3-7 Days
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WB(1)
IHC(3)
Assay(1)
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Also for IRF4 (NM_002460)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the N-terminus in human IRF-4 was used as an immunogen.
Clone NameEPR5653 IsotypeTissue culture supernatant
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, ASSAY, IHC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250
BufferDoes not react with Mouse, Rat. Is unsuitable for Flow Cyt or ICC.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt or ICC.

Reference Data

Target NameHomo sapiens interferon regulatory factor 4 (IRF4), transcript variant 1
Alternative NameLSIRF; MUM1; NF-EM5
Database LinkNP_002451
FunctionIRF-4 belongs to the IRF (interferon regulatory factor) family of transcription factors, characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by viruses and the regulation of interferon-inducible proteins.IRF-4 is lymphocyte specific and negatively regulates Toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A mutation in IRF-4 may be a cause of multiple myeloma (1).
Related Pathway
Apoptosis

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WB Image
Western blot - Anti-MUM1 antibody [EPR5653]; All lanes : Anti-MUM1 antibody [EPR5653] at 1/1000 dilution.Lane 1 : Ramos cell lysate.Lane 2 : HuT 78 cell lysate.Lane 3 : IM 9 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 52 kDa.
Assay Image
Other-Anti-MUM1 antibody [EPR5653](TA310950); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [EPR5653]; TA310950, at 1/100, staining MUM1 in paraffin-embedded Human colon tissue by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [EPR5653]; TA310950, at 1/100, staining MUM1 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUM1 antibody [EPR5653]; Immunohistochemical analysis of dog spleen tissue, staining MUM1 with TA310950.Tissue was fixed with formaldehyde and blocked with 2.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a TRIS-EDTA buffer (pH 9). Samples were incubated with primary antibody (1/50 in PBS plus 1X casein) for 1 hour 30 minutes at 37°C. An undiluted HRP-conjugated horse anti-rabbit polyclonal IgG was used as the secondary antibody.

 

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