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Anti-IRF3 TRUEMAB Antibody Clone OTI2G3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500520 anti-IRF3 mouse monoclonal antibody, clone OTI2G3 (formerly 2G3) 100ul $325 In Stock
LC400600 IRF3 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500520 Carrier-free (BSA/glycerol-free) IRF3 mouse monoclonal antibody, clone OTI2G3 (formerly 2G3) 100ug $450 3-4 weeks
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WB(1)
IHC(7)
IF(1)

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human IRF3 expression vector
Clone NameClone OTI2G3 IsotypeIgG2a
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100,
Predicted MW Explanation 47.2 KDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens interferon regulatory factor 3 (IRF3), transcript variant 1
Alternative NameMGC94729; interferon regulatory factor 3
Database LinkNP_001562
Entrez Gene 3661 Human
FunctionIRF3 encodes interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes.
Related PathwayTranscription FactorsDruggable Genome Toll-like receptor signaling pathwayRIG-I-like receptor signaling pathwayCytosolic DNA-sensing pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF3 (RC209951, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF3.
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Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-IRF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500520, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-IRF3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500520, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500520, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500520, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500520, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500520, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-IRF3mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500520, Dilution 1:50)
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Anti-IRF3 mouse monoclonal antibody (TA500520) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IRF3(RC209951).
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