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Anti-IRF3 Antibody EPR2418Y
Also for IRF3 (NM_001571)
|A synthetic peptide corresponding to residues surrounding the N-terminus of human IRF-3 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; FC: 1:20 - 1:100
|Does not react with Rat. Is unsuitable for IP.|
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens interferon regulatory factor 3 (IRF3), transcript variant 1|
|MGC94729; interferon regulatory factor 3|
|A family of interferon (IFN) regulatory factors (IRFs) have been shown to play a role in transcription of IFN genes as well as IFN-stimulated genes. The IRF-3 gene encodes a 50-kDa protein that binds specifically to the IFN-stimulated response element (ISRE) but not to the IRF-1 binding site PRD-I. Overexpression of IRF-3 stimulates expression of the IFN-stimulated gene 15 (ISG15) promoter, an ISRE-containing promoter. he high amino acid homology between IRF-3 and ISG factor 3 gamma polypeptide (ISGF3 gamma) and their similar binding properties indicate that, like ISGF3 gamma, IRF-3 may activate transcription by complex formation with other transcriptional factors, possibly members of the Stat family (1). IRF-3 is expressed constitutively in a variety of tissues, and the relative levels of IRF-3 mRNA do not change in virus-infected or IFN-treated cells. (2). In uninfected cells, the IRF-3 component of DRAF1 resides in the cytoplasm. The cytoplasmic localization of IRF-3 is dependent on a nuclear export signal, and IRF-3 is recognized by the chromosome region maintenance 1 (CRM1) (also known as exportin 1) shuttling receptor. Following infection and specific phosphorylation, IRF-3 accumulates in the nucleus where it associates with CBP and p300 (3). |
Toll-like receptor signaling pathway
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Western blot - IRF3 antibody [EPR2418Y]; All lanes : Anti-IRF3 antibody [EPR2418Y] at 1/2000 dilution.Lane 1 : U937 cell lysate.Lane 2 : 293T cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : MCF7 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.goat anti-rabbit HRP conjugated, at 1/2000 dilution.Predicted band size : 47 kDa.Observed band size : 47 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - IRF3 antibody [EPR2418Y]; ab68481 at 1/100 dilution staining IRF3 in human tonsil by Immunohistochemistry, Paraffin-embedded tissue.
Flow Cytometry - Anti-IRF3 antibody [EPR2418Y]; Overlay histogram showing HeLa cells stained with ab68481 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.