Also for IREB2 (NM_004136)
|A synthetic peptide made to an internal portion of the murine IRP2 protein sequence (between residues 100-200). UniProt Q811J3|
|Human, Mouse, Primate, Rat
|Tris-glycine and 150mM NaCl and 0.05% Sodium Azide|
|Peptide affinity purified
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Raw macrophages (mouse) were either left untreated (lane 1) or treated overnight with 100mM DFO (lane 2) or 100mM hemin (lane 3). Proteins extracts were subjected to western blotting using IRP2 or b-actin antibodies diluted 1:500 in TBS-Tween.
Detection of IRP2 in mouse liver lysate using anti-IRP2.