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Anti-ING3 Antibody

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SKU Description Amount Price Availability*  
TA319233
  • Goat polyclonal anti-p47 ING3 antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC412783) , 20ug
100ug 325 3-7 Days
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WB(1)
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Also for ING3 (NM_019071)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole Goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 294-304 of Human ING3 protein (Inhibitor of growth family, member 3). This sequence only shows homology to isoform 1 for ING3.
Clone Name Isotype
Species Reactivityhuman Concentration1.10 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:10,000 - 1:40,000, WB: 1:200 - 1:2,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note p47 ING3 is a tumor suppressor protein similar to ING1 that  can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Over-expression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Multiple alternatively spliced transcript variants have been observed.  The accession number listed below is for variant (1) that encodes the longest isoform.

Reference Data

Target NameHomo sapiens inhibitor of growth family, member 3 (ING3), transcript variant 1
Alternative NameEaf4; ING2; MEAF4; p47ING3
Database LinkNP_061944
Function
Related Pathway

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WB Image
Western blot using purified anti-ING3 antibody shows detection of a band at ~55 kDa corresponding to ING3 in RKO cells transfected with ING3 (lane 2). Control RKO cells do not show detection of this specific band (lane 1). The identity of the non-specific bands at 33 kDa and 20 kDa has not been determined. Each lane contains approximately 10 µg of RKO whole cell lysate (ATCC# CRL-2577 - human colon cancer) separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose. After blocking with 5% NF dry milk, the membrane was probed with the primary antibody diluted to 1:1,000. Incubation was at 4° C overnight followed by washes and reaction with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MXHu (605-432-013) for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

 

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