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Anti-ILK Antibody EP1593Y
|A synthetic peptide corresponding to residues near N-term of human ILK protein was used as immunogen|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:2000 - 1:10000; IP: 1:30; ICC: 1:100 - 1:250; FC: 1:100; IHC-P: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens integrin-linked kinase (ILK), transcript variant 1|
|ILK-1; ILK-2; P59; p59ILK|
|Integrin-linked kinase (ILK) is a key cytoplasmic plaque protein involved in the mediation of integrin functions. It plays a key role as a regulator during cell survival, cell proliferation, cell migration, and cell to cell adhesion (1). ILK links integrins to the actin cytoskeleton, allowing transduction of signals through integrins to the extracellular matrix (ECM). Also, ILK has been linked to the regulation of smooth contraction and cell motility in non-muscle cells through phosphorylation of myosin (2). Irregular activity level of ILK has been linked to expression of certain tumor. ILK overexpression caused by inactivating mutation in tumor suppressors (e.g. PTEN and APC) lead to tumor formation in transgenic mouse. Up-regulated ILK expression and activity are seen in Ewings scaromas, colorectal cancers, and late staged prostate cancers (3). |
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Western blot - Integrin linked ILK antibody [EP1593Y]; Anti-Integrin linked ILK antibody [EP1593Y] at 1/2000 dilution + COS-1 cell lysate at 10 µg.Secondary.Goat anti-rabbit HRP labeled at 1/2000 dilution.Predicted band size : 51 kDa.Observed band size : 51 kDa.
Western blot - Integrin linked ILK antibody [EP1593Y]; All lanes : Anti-Integrin linked ILK antibody [EP1593Y] at 1/2000 dilution.Lane 1 : Rat Brain.Lane 2 : Rat Heart.Lane 3 : Rat Kidney.Lane 4 : Rat Spleen.Predicted band size : 51 kDa.Exposure time : 3 minutes
Western blot - Integrin linked ILK antibody [EP1593Y]; Anti-Integrin linked ILK antibody [EP1593Y] + PC12.Predicted band size : 51 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Integrin linked ILK antibody [EP1593Y]; Immunohistochemical analysis of paraffin-embedded human heart muscle using ab52480 at a 1/50 dilution.
Flow Cytometry - Anti-Integrin linked ILK antibody [EP1593Y]; Overlay histogram showing HEK293 cells stained with ab52480 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.