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Home Antibody All anti-IL6 antibodies
UltraMAB

Anti-IL6 GAT Antibody Polyclonal

Generated by Genomic Immunization

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA590788 Rabbit Polyclonal IL6 Antibody 50ug $325 In Stock
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WB(1)
IHC(1)
ELISA(1)

OriGene Data

ImmunogenDNA immunization. This antibody is specific for the Middle Region of the target protein.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman Concentration1.01mg/ml
Guaranteed Application *WB, ELISA, IHC Suggested Dilutions WB 1:20000, IHC 1:150,ELISA 1:100-1:2000
Buffer20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0
Purification The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
Note This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT

Reference Data

Target NameHomo sapiens interleukin 6 (IL6)
Alternative NameBSF2; HGF; HSF; IFNB2; IL-6
Database LinkNP_000591
Entrez Gene 3569 Human
FunctionThe antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts). In Western blot analysis of natural cell products or human body fluids, multiple bands of IL-6 will appear due to the variable amount of glycosylation on the molecule (21-28 kDa). The antiserum is also useful for neutralization of human IL-6 activity in bioassays. For neutralization, incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG (heat inactivated) is recommended. In neutralization experiments in vitro, this antibody does not result in enhanced activity of IL-6. However, because antibodies to IL-6 may act as a soluble receptor in vivo, some antibodies to IL-6 act as carriers and enhance IL-6 activity.
Related PathwaySecreted ProteinDruggable Genome Cytokine-cytokine receptor interactionToll-like receptor signaling pathwayNOD-like receptor signaling pathwayCytosolic DNA-sensing pathwayJak-STAT signaling pathwayHematopoietic cell lineageMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IL6 (RC202078, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IL6.
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ELISA: IL6 Antibody
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IL6 Rabbit Polyclonal antibody. (TA590788)
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