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Also for IL1R2 (NM_001261419)
|This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-1R type II.|
||ELISA: 1:1,000 - 1:2,000, WB: 1:500 - 1:2,000, IP: 1:400 - 1:800
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Homo sapiens interleukin 1 receptor, type II (IL1R2), transcript variant 3|
|CD121b; CDw121b; IL-1R-2; IL-1RT-2; IL-1RT2; IL1R2c; IL1RB|
Cytokine-cytokine receptor interaction
Hematopoietic cell lineage
MAPK signaling pathway
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Western blot using anti-IL-1RII antibody. This blot shows detection of an IL-1RII-GST fusion protein (~500 ng, lane 1, green, 800 nm channel). Minimal reactivity is observed against GST (data not shown). Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000. Incubation was for 2 hrs at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (611-132-122) for 45 min at room temperature. Molecular weight markers are shown for size comparison (lane M, red, 700 nm channel). IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.