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Anti-IL1B Antibody

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SKU Description Amount Price Availability*  
TA319141 Rabbit polyclonal IL-1 beta antibody 1mg $325 3-7 Days
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WB(1)

OriGene Data

ImmunogenThis antibody was prepared by repeated immunizations with recombinant human IL-1ß produced in E.coli.  The MW of the recombinant 153 aa IL-1ß was 17 kDa with the N-terminal amino acid at position alanine 117.  This cleavage site is generated by the IL-1ß converting enzyme (ICE, capase-1).
Clone Name IsotypeIgG
Species Reactivityhuman, primate, dog Concentration2.0 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:500 - 1:2,000, WB: 1:1,000, IHC: 1:100 - 1:200, IP: 1:400 - 1:800
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note IL-1 beta (also known as Interleukin-1 beta, IL-1ß and catabolin) is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1ß is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

IL-1B was used at a 1:200 dilution incubated 1 h at RT to detect dog IL-1B by WB. Lane 1 and 2 were loaded with 2.5 ug and 500 ng of dog IL-1B respectively. The molecular weight of the detected band is estimated by comparison to molecular weight markers (not shown). Detection occurred using a 1:3,000 dilution of IRDye™800 conjugated Donkey anti-Rabbit IgG.
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