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Home Antibody All anti-IL17a antibodies

Anti-IL17a Antibody 20B4.G10.F5

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SKU Description Amount Price Availability*  
TA319587
  • Rat monoclonal Mouse IL-17A antibody
100ug $325 3-7 Days
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WB(1)
FC(2)
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Also for IL17a (NM_010552)
cDNA Clone shRNA/siRNA Primer Pair Protein Antibody

OriGene Data

ImmunogenThis Protein A purified monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse IL-17A protein (produced in E.coli) followed by hybridoma development.
Clone Name20B4.G10.F5 Isotype
Species ReactivityMouse Concentration2.94 mg/mL
Guaranteed Application *WB, FC Suggested DilutionsELISA: 1:10,000, WB: 1:1000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Mouse Interleukin-17A (IL-17A), also known as CTLA-8, is a proinflammatory cytokine member of a six-species family of proteins (IL-17A-17F). Mouse IL-17A protein is a homodimer consisting of two 134 amino acids peptides. IL-17A is secreted mainly by activated CD4+ and CD8+ T lymphocytes and acts through its receptor, IL-17R, to induce the expression of many mediators of inflammation, most strikingly, those that are involved in the proliferation, maturation and chemotaxis of neutrophils. Elevated levels of IL-17A have been associated with several conditions, including rheumatoid arthritis, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer and multiple sclerosis. There is 58% identity between the amino acid sequence of human and mouse IL-17A. Anti-IL-17A antibody is ideal for investigators involved in Immunology research.

Reference Data

Target NameMus musculus interleukin 17A (Il17a)
Alternative NameCtla-8; Ctla8; IL-17; IL-17A; Il17
Database LinkNP_034682
Function
Related Pathway

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WB Image
Western Blot showing detection of Mouse IL-17A. 100 ng of Mouse IL-17A was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20°C, Anti-Mouse IL-17A (RAT) Antibody (p/n TA319587) was used at 1:1000 in 1% BSA-TTBS over night at 4°C. Peroxidase conjugated Rabbit Anti-mouse secondary antibody (p/n 610-4302) was diluted in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) at 1:40,000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc® 4000 MP. Band indicates correct 23 kDa molecular weight position expected for Mouse IL-17A.
FC Image
monoclonal anti IL17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 µg/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 µg/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 µg/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (TA319587) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for positive and negative controls.
FC Image
monoclonal anti IL-17A was used to detect IL-17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 µg/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 µg/mL anti-mouse IFN? over 8-10 days of culture. Cells were incubated for 15-20 minutes with Add rat anti-mouse CD4 APC at a concentration of 0.125 µg/mL, washed, fixed and permeabilized and incubated with Rat anti mouse IL-17A monoclonal Antibody (TA319587) or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for Rockland’s monoclonal anti mouse IL-17A antibody (TA319587)

 

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