Home Antibody All anti-IL17A antibodies
Also for IL17A (NM_001106897)
|This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant rat IL17-A protein.|
||ELISA: 1:20,000-1:100,000, WB: 1:2,000-1:10,000, IHC: 1:1,000-1:5,000
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Rat IL17-A (also known as Interleukin-17, Cytotoxic T-lymphocyte-associated antigen 8 and CTLA-8) is a proinflammatory cytokine member of a six-species family of proteins (IL-17A-17F). Rat IL-17A protein is a homodimer consisting of two 134 amino acids peptides. IL-17A is secreted mainly by activated CD4+ and CD8+ T lymphocytes and acts through its receptor, IL-17R, to induce the expression of many mediators of inflammation, most strikingly, those that are involved in the proliferation, maturation and chemotaxis of neutrophils. Elevated levels of IL-17A have been associated with several conditions, including rheumatoid arthritis, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer and multiple sclerosis. There is 58% identity between the amino acid sequence of human and rat IL-17A. Anti-IL-17A antibody is ideal for investigators involved in Immunology research.
|Rattus norvegicus interleukin 17A (Il17a)|
|CTLA-8; IL-17; IL-17A; Il17|
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Western Blot showing detection of Rat IL-17A. 50ng of Rat IL-17A (Lane 1) was run on a 4-20% gel and transferred to 0.45 Âµm nitrocellulose. After blocking with 5% Blotto (p/n B501-0500) 30 min at 20Â°C, Anti-Rat IL-17A (RABBIT) Antibody Biotin Conjugated (p/n TA319353) secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070). HRP Streptavidin (p/n S000-03) was used at 1:40,000 in MB-070 for 30 min at 20Â°C and imaged using the Bio-Rad VersaDocÂ® 4000 MP. Arrow indicates correct 15 kDa molecular weight position expected for Rat IL-17A.