Anti-IDS Antibody, Clone 4G2

Also for IDS (NM_000202)

OriGene Data

Immunogen	Full length human recombinant protein of human IDS(NP_000193) produced in HEK293T cell.
Clone Name	Clone 4G2	Isotype	IgG2a
Species Reactivity	Human , Dog , Rat , Monkey , Mouse	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens iduronate 2-sulfatase (IDS), transcript variant 1
Alternative Name	MPS2; SIDS
Database Link	NP_000193
Function	Iduronate-2-sulfatase is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this X-chromosome gene that result in enzymatic deficiency lead to the sex-linked Mucopolysaccharidosis Type II, also known as Hunter Syndrome. Iduronate-2-sulfatase has a strong sequence similarity with human arylsulfatases A, B, and C, and human glucosamine-6-sulfatase. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq].
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDS (RC219187, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDS.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IDS monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-IDS mouse monoclonal antibody. (TA504277)

IHC Image

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-IDS mouse monoclonal antibody. (TA504277)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-IDS mouse monoclonal antibody. (TA504277)

IHC Image

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-IDS mouse monoclonal antibody. (TA504277)

IHC Image

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-IDS mouse monoclonal antibody. (TA504277)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-IDS mouse monoclonal antibody. (TA504277)

IHC Image

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-IDS mouse monoclonal antibody. (TA504277)

IHC Image

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-IDS mouse monoclonal antibody. (TA504277)

IF Image

Anti-IDS mouse monoclonal antibody (TA504277) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IDS(RC219187).

FC Image

HEK293T cells transfected with either RC219187 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-IDS antibody(TA504277), and then analyzed by flow cytometry.

FC Image

Flow cytometric Analysis of Hela cells, using anti-IDS antibody(TA504277),(Red), compared to a nonspecific negative control antibody,(Blue).

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-IDS antibody(TA504277),(Red), compared to a nonspecific negative control antibody,(Blue).

Anti-IDS TrueMAB Antibody Clone 4G2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data