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Anti-IDH3A TRUEMAB Antibody Clone 2F11
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for IDH3A (NM_005530)
|Full length human recombinant protein of human IDH3A (NP_005521) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens isocitrate dehydrogenase 3 (NAD+) alpha (IDH3A)|
|H-IDH alpha; NAD(H)-specific isocitrate dehydrogenase alpha subunit; NAD+-specific ICDH; isocitrate dehydrogenase (NAD+) alpha chain; isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; isocitric dehydrogenase; isocitrate dehydrogenase 3 (NAD+) a|
|Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDH3A (RC200313, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDH3A.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IDH3A monoclonal antibody.
Immunohistochemical staining of paraffin-embedded liver tissue using anti-IDH3Amouse monoclonal antibody. (TA500739, Dilution 1:50)
Anti-IDH3A mouse monoclonal antibody (TA500739) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IDH3A(RC200313).
Immunofluorescent staining of HepG2 cells using anti-IDH3A mouse monoclonal antibody (TA500739).
Flow cytometric analysis of Hela cells, using anti-IDH3A antibody(TA500739),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
Flow cytometric analysis of Jurkat cells, using anti-IDH3A antibody(TA500739),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).