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Home Antibody All anti-IDH3A antibodies

Anti-IDH3A TRUEMAB Antibody Clone OTI2F11

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (1) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500739 anti-IDH3A mouse monoclonal antibody, clone OTI2F11 (formerly 2F11) 100ul $379 In Stock
LC401698 IDH3A HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500739 Carrier-free (BSA/glycerol-free) IDH3A mouse monoclonal antibody, clone OTI2F11 (formerly 2F11) 100ug $450 3-4 weeks

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(2)
IHC(1)
IF(2)
FC(2)
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Also for IDH3A (NM_005530)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human IDH3A (NP_005521) produced in HEK293T cell.
Clone NameClone OTI2F11 IsotypeIgG2a
Species ReactivityHuman, Dog Concentration0.55 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
Predicted MW Explanation 39.6 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens isocitrate dehydrogenase 3 (NAD+) alpha (IDH3A)
Alternative NameH-IDH alpha; NAD(H)-specific isocitrate dehydrogenase alpha subunit; NAD+-specific ICDH; isocitrate dehydrogenase (NAD+) alpha chain; isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; isocitric dehydrogenase; isocitrate dehydrogenase 3 (NAD+) a
Database LinkNP_005521
Entrez Gene 3419 Human
Entrez Gene 479066 Dog
FunctionIsocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.
Related Pathway Citrate cycle (TCA cycle)Metabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDH3A (RC200313, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDH3A.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IDH3A monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-IDH3Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500739, Dilution 1:50)
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Anti-IDH3A mouse monoclonal antibody (TA500739) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IDH3A(RC200313).
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Immunofluorescent staining of HepG2 cells using anti-IDH3A mouse monoclonal antibody (TA500739).
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Flow cytometric analysis of Hela cells, using anti-IDH3A antibody(TA500739),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
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Flow cytometric analysis of Jurkat cells, using anti-IDH3A antibody(TA500739),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
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