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Anti-IDH1 TRUEMAB Antibody clone 2H9
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for IDH1 (NM_005896)
|Protein expressed in 293T cell transfected with human IDH1 expression vector|
|WB, IHC, IF
||WB 1:2000, IHC 1:50, IF 1:100,
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), transcript variant 1|
|IDCD; IDH; IDP; IDPC; PICD|
|Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDH1 (RC210582, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDH1 .
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IDH1 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded breast tissue using anti-IDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500610, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded colon tissue using anti-IDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500610, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded endometrium tissue using anti-IDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA500610, Dilution 1:50)
Anti-IDH1 mouse monoclonal antibody (TA500610) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IDH1 (RC210582).