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Anti-ID3 TRUEMAB Antibody clone 4D1
TrueMAB Antibodies - Made against Authentic Protein Antigens
|Full length human recombinant protein of human ID3 (NP_002158) produced in HEK293T cell.|
|WB, IF, FC
||WB 1:1000, IF 1:100, FLOW 1:100,
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens inhibitor of DNA binding 3, dominant negative helix-loop-helix protein (ID3)|
|Members of the ID family of helix-loop-helix (HLH) proteins lack a basic DNA-binding domain and inhibit transcription through formation of nonfunctional dimers that are incapable of binding to DNA.[supplied by OMIM]. |
TGF Beta Signaling Pathway
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID3 (RC200583, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ID3.
Anti-ID3 mouse monoclonal antibody (TA500715) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ID3(RC200583).
Flow cytometric Analysis of Hela cells, using anti-ID3 antibody(TA500715),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-ID3 antibody(TA500715),(Red), compared to a nonspecific negative control antibody,(Blue).