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Anti-ICAM-1 Antibody EPR4776
Also for ICAM-1 (NM_000201)
|A recombinant protein corresponding to a fragment near the C-terminus of human ICAM-1 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; FC: 1:100 - 1:500
|Does not react with Mouse, Rat. Is unsuitable for ICC.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for ICC.
|Homo sapiens intercellular adhesion molecule 1 (ICAM1)|
|BB2; CD54; P3.58|
|Antigen-specific cell contacts in the immune system are strengthened by antigen-nonspecific interactions, mediated in part by lymphocyte-function associated (LFA) antigens. ICAM-1 (intercellular adhesion molecule-1) has been defined as a ligand for LFA-1. Monoclonal antibodies to ICAM-1 block T lymphocyte adhesion to fibroblasts and endothelial cells; they disrupt the interaction between cytotoxic T cells and target cells (1). The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. However, ICAM-1 is also used as a receptor by the major group of human rhinoviruses and is a catalyst for the subsequent viral uncoating during cell entry (2). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells; its expression is regulated by inflammatory cytokines (1).|
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Western blot - ICAM1 antibody [EPR4776]; All lanes : Anti-ICAM1 antibody [EPR4776] at 1/1000 dilution.Lane 1 : Raji cell lysate.Lane 2 : Ramos cell lysate.Lane 3 : A549 cells treated with TNF-alpha, lysate.Lane 4 : A549 cell lysate.Lane 5 : HUVEC cells treated with TNF-alpha, lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 58 kDa.Observed band size : 89 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ICAM1 antibody [EPR4776]; ab109361, at 1/100, staining ICAM1 in Human kidney tissue by immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ICAM1 antibody [EPR4776]; , at 1/100, staining ICAM1 in Human tonsil tissue by immunohistochemistry.
Flow Cytometry - Anti-ICAM1 antibody [EPR4776]; Overlay histogram showing Ramos cells stained with ab109361 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.