Home Antibody All anti-ICAM-1 antibodies
Anti-ICAM-1 Antibody EPR4776
Also for ICAM-1 (NM_000201)
|A recombinant protein corresponding to a fragment near the C-terminus of human ICAM-1 was used as an immunogen.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; FC: 1:100 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for ICC.
|Homo sapiens intercellular adhesion molecule 1 (ICAM1)|
|BB2; CD54; P3.58|
Entrez Gene 3383 Human
|Antigen-specific cell contacts in the immune system are strengthened by antigen-nonspecific interactions, mediated in part by lymphocyte-function associated (LFA) antigens. ICAM-1 (intercellular adhesion molecule-1) has been defined as a ligand for LFA-1. Monoclonal antibodies to ICAM-1 block T lymphocyte adhesion to fibroblasts and endothelial cells; they disrupt the interaction between cytotoxic T cells and target cells (1). The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. However, ICAM-1 is also used as a receptor by the major group of human rhinoviruses and is a catalyst for the subsequent viral uncoating during cell entry (2). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells; its expression is regulated by inflammatory cytokines (1).|
|ES Cell Differentiation/IPSTransmembraneDruggable Genome Cell adhesion molecules (CAMs)Natural killer cell mediated cytotoxicityLeukocyte transendothelial migrationViral myocarditis|
* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
Western blot - ICAM1 antibody [EPR4776]; All lanes : Anti-ICAM1 antibody [EPR4776] at 1/1000 dilution.Lane 1 : Raji cell lysate.Lane 2 : Ramos cell lysate.Lane 3 : A549 cells treated with TNF-alpha, lysate.Lane 4 : A549 cell lysate.Lane 5 : HUVEC cells treated with TNF-alpha, lysate.Lysates/proteins at 10 ug per lane.Predicted band size : 58 kDa.Observed band size : 89 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ICAM1 antibody [EPR4776]; TA307840, at 1/100, staining ICAM1 in Human kidney tissue by immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ICAM1 antibody [EPR4776]; , at 1/100, staining ICAM1 in Human tonsil tissue by immunohistochemistry.
Flow Cytometry - Anti-ICAM1 antibody; Overlay histogram showing Ramos cells stained with TA307840 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) used under the same conditions. Unlabelled sample (blue line) was also used as a control. This antibody gave a positive signal in Ramos cells under the same conditions.