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Anti-HSPH1 Antibody EPR4577
Also for HSPH1 (NM_006644)
|A synthetic peptide corresponding to residues in human HSP105 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens heat shock 105kDa/110kDa protein 1 (HSPH1), transcript variant 1|
|HSP105; HSP105A; HSP105B; NY-CO-25|
|HSP105α and HSP105beta are mammalian members of the HSP105/110 family, a diverged subgroup of the HSP70 family (1). It has been reported that two isoforms of HSP105 (HSP105α and HSP105beta) were both moderately expressed in adrenal, spleen, liver and heart, and both increased markedly after heat shock. However, in brain HSP105α was characteristically highly expressed over HSP105beta, but neither increased after heat shock (2). HSP105 prevents the aggregation of denatured proteins in cells under severe stress, on which the ATP levels decrease markedly, and it inhibits HSPA8/HSC70 ATPase and chaperone activities (3).|
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Western blot - Hsp105 antibody [EPR4577]; All lanes : Anti-Hsp105 antibody [EPR4577] at 1/10000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HepG2 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 97 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hsp105 antibody [EPR4577]; ab108625 at 1/100 dilution staining Hsp105 in Human testis by Immunohistochemistry, Paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - Hsp105 antibody [EPR4577]; ab108625 at 1/100 dilution staining Hsp105 in HeLa cells by Immunofluorescence.