Home Antibody All anti-HSP90AB1 antibodies
Also for HSP90AB1 (NM_007355)
|The antiserum was produced against synthesized non-phosphopeptide derived from human HSP90B around the phosphorylation site of serine 254 (V-G-SP-D-E).|
|Human, Mouse, Rat
|WB, IHC, IF
||WB: 1:500~1000, IHC: 1:50~100, IP: Various DilutionIF: 1:100~1:500, ELISA: 1:40000
|Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (Protein A or G Sepharose)
|HSP90B (Ab-254) antibody detects endogenous levels of total HSP90B protein.
|Homo sapiens heat shock protein 90kDa alpha (cytosolic), class B member 1 (HSP90AB1), transcript variant 2|
|D6S182; HSP84; HSP90B; HSPC2; HSPCB|
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Western blot analysis of extracts from Hela cells treated with TNF-a (20ng/ml, 30min), using HSP90B (Ab-254) antibody (#B0013, Line 1 and 2).The lane on the right is treated with the synthesized peptide.Western blot analysis of extracts from HeLa cells (Lane 2), CoLo cells (Lane 3), HepG2 cells (Lane 4) and 293 cells (Lane 5), using HSP90B (Ab-254) Antibody. The lane on the left is treated with synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSP90B (Ab-254) antibody (#B0013).The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, treated with TNF-a (20nM, 15mins), using HSP90B (Ab-254) antibody (#B0013).The picture on the right is treated with the synthesized peptide.