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Anti-HSD17B1 Antibody EP1682Y
Also for HSD17B1 (NM_000413)
|A synthetic peptide corresponding to residues on the C-terminus of human HSD17B1 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:10000 - 1:50000; ICC/IF: 1:100 - 1:250; FC: Use 1?g for 106<:sup> cells; IP: 1:100; IHC-P: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens hydroxysteroid (17-beta) dehydrogenase 1 (HSD17B1)|
|EDH17B2; EDHB17; HSD17; SDR28C1|
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Western blot - HSD17B1 antibody [EP1682Y]; Anti-HSD17B1 antibody [EP1682Y] at 1/100000 dilution + Human placenta lysate at 10 µg.Secondary.Goat anti-Rabbit HRP labeled. at 1/2000 dilution.Predicted band size : 35 kDa.Observed band size : 35 kDa.
Immunohistochemistry (Paraffin-embedded sections) - HSD17B1 antibody [EP1682Y]; Ab51045 (1/100 dilution) staining human HSD17B1 in paraffin embedded human placenta tissue by Immunohistochemistry.
Flow Cytometry - Anti-HSD17B1 antibody [EP1682Y]; Overlay histogram showing JEG3 cells stained with ab51045 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells ) used under the same conditions. Acquisition of >5,000 events was performed.