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Anti-HSCB TRUEMAB Antibody Clone OTI3E1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA507274 HSCB mouse monoclonal antibody, clone OTI3E1 (formerly 3E1) 100ul $325 In Stock
LC406803 HSCB HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF507274 Carrier-free (glycerol/BSA-free) HSCB mouse monoclonal antibody, clone OTI3E1 (formerly 3E1) 100ug $450 3-4 Days
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WB(2)
IHC(6)

OriGene Data

ImmunogenFull length human recombinant protein of human HSCB(NP_741999) produced in HEK293T cell
Clone NameClone OTI3E1 IsotypeIgG1
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration1 mg/ml
Guaranteed Application *WB, IHC Suggested Dilutions WB 1:400~4000, IHC 1:150,
Predicted MW Explanation 27.2 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens HscB mitochondrial iron-sulfur cluster co-chaperone (HSCB)
Alternative NamedJ366L4.2; DNAJC20; HSC20; JAC1
Database LinkNP_741999
Entrez Gene 150274 Human
Entrez Gene 100900 Mouse
Entrez Gene 360826 Rat
Entrez Gene 713734 Monkey
Entrez Gene 477531 Dog
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSCB (RC200843, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSCB.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSCB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-HSCB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507274)
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HSCB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507274)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HSCB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507274)
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-HSCB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507274)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HSCB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507274)
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-HSCB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA507274)
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