Home Antibody All anti-HMOX1 antibodies
Anti-HMOX1 Antibody EP1391Y
Also for HMOX1 (NM_002133)
|A synthetic peptide corresponding to residues near the C-terminus of human HO-1 was used as an immunogen.|
|Mouse, Guinea pig, Human (Does not react with: Rat)
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:2000; IP: 1:60; FC: 1:1000; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Does not react with Rat
|Homo sapiens heme oxygenase (decycling) 1 (HMOX1)|
|bK286B10; HMOX1D; HO-1; HSP32|
Entrez Gene 3162 Human
Entrez Gene 15368 Mouse
|Stressed mammalian cells up-regulate heme oxygenase 1 (HO-1), which catabolizes heme to biliverdin, carbon monoxide, and free iron. Results provide genetic evidence that up-regulation of HO-1 serves as an adaptive mechanism to protect cells from oxidative damage during stress (1). When cells are injured they release their contents, resulting in a local accumulation of free heme proteins and heme. HO-1 plays a crucial role in the inflammatory process during wound healing (2). TNFalpha accelerates inflammatory responses by down-regulating HO-1 expression in human monocytes. TNF antagonists may block this TNF-dependent suppression of HO-1 expression, resulting in an amelioration of inflammation (3). |
|TransmembraneDruggable Genome Porphyrin and chlorophyll metabolism|
* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
Western blot - Heme Oxygenase 1 antibody [EP1391Y]; Anti-Heme Oxygenase 1 [EP1391Y] antibody at 1/2000 dilution + fetal liver lysate at 10 ug.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 33 kDa.Observed band size : 33 kDa.
Immunohistochemistry (Paraffin-embedded sections) - Heme Oxygenase 1 antibody [EP1391Y]; TA300823 at 1/100-1/250 dilution staining Heme Oxygenase 1 in human liver by Immunohistochemistry, Paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - Heme Oxygenase 1 antibody [EP1391Y]; TA300823 at 1/250-1/500 dilution staining Heme Oxygenase 1 in HeLa cells by Immunofluorescence.
Flow Cytometry - Anti-Heme Oxygenase 1 antibody; Overlay histogram showing HEK293 cells stained with TA300823 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) used under the same conditions. Unlabelled sample (blue line) was also used as a control. This antibody gave a positive signal in HEK293 cells under the same conditions.