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Anti-HLA Antibody EPR1394Y
|A synthetic peptide corresponding to residues in human MHC class 1 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:20
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens major histocompatibility complex, class I, A (HLA-A), transcript variant 1 (A*03:01:0:01 allele)|
|HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq]. |
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Western blot - Anti-MHC class I + HLA B [EPR1394Y] antibody; All lanes : Anti-MHC class I + HLA B [EPR1394Y] antibody at 1/1000 dilution.Lane 1 : Raij cell lysate.Lane 2 : THP1 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 41 kDa.Observed band size : 41 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MHC class I + HLA B [EPR1394Y] antibody; Immunohistochemical analysis of paraffin-embedded Human tonsil labelling MHC class I with ab134189 at 1/100 dilution.