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Anti-HLA-DQA1 Antibody EPR7300
Also for HLA-DQA1 (NM_002122)
|A synthetic peptide corresponding to residues on the C-terminus in human HLA-DQA1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, ASSAY, IHC
||WB: 1:10000 - 1:50000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or ICC/IF.
|Homo sapiens major histocompatibility complex, class II, DQ alpha 1 (HLA-DQA1)|
|CD; CELIAC1; DQ-A1; GSE; HLA-DQA|
|HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules (1).|
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Western blot - Anti-HLA-DQA1 antibody [EPR7300]; All lanes : Anti-HLA-DQA1 antibody [EPR7300] at 1/10000 dilution.Lane 1 : Human lymph node lysate.Lane 2 : Daudi cell lysate .Lane 3 : Human spleen lysate .Lane 4 : Raji cell lysate .Lane 5 : Human tonsil lysate .Lysates/proteins at 10 µg per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 28 kDa.Observed band size : 33-35 kDa .
Other-Anti-HLA-DQA1 antibody [EPR7300](ab128959); Equilibrium disassociation constant (KD)..
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DQA1 antibody [EPR7300]; ab128959, at 1/100 dilution, staining HLA-DQA1 in paraffin-embedded Human colon tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HLA-DQA1 antibody [EPR7300]; ab128959, at 1/100 dilution, staining HLA-DQA1 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.