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Home Antibody All anti-HIST2H4B antibodies

Anti-HIST2H4B Antibody EP1000Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303817
  • Rabbit monoclonal antibody against Histone H4, Acetyl-K5(EP1000Y)
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
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Also for HIST2H4B (NM_001034077)
cDNA Clone shRNA/siRNA Primer Pair Protein Request Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human Histone H4 was used as an immunogen
Clone NameEP1000Y IsotypeIgG
Species ReactivityMouse, Rat, Human, Xenopus laevis, Rice ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsChIP: Use at an assay dependent dilution; WB: 1:10000 - 1:50000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250 - 1:500; IP: 1:50
Predicted MW Explanation 11 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens histone cluster 2, H4b (HIST2H4B)
Alternative NameH4/o
Database LinkNP_001029249
Entrez Gene 554313 Human
FunctionHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy. [provided by RefSeq].
Related Pathway Systemic lupus erythematosus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Histone H4 (acetyl K5) antibody [EP1000Y]; All lanes : Anti-Histone H4 (acetyl K5) [EP1000Y] antibody - ChIP Grade at 1/1000000 dilution.Lane 1 : Untreated HeLa cells.Lane 2 : TSA treated HeLa calls.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP labelled (1:2000).Predicted band size : 11 kDa.Observed band size : 11 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Histone H4 (acetyl K5) antibody [EP1000Y]; Ab51997 (1:500) staining human histone H4 on human brain glioma by immunohistochemistry using paraffin embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Histone H4 (acetyl K5) antibody [EP1000Y] - ChIP Grade; ICC/IF image of TA303817 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.

 

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