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Anti-HIST2H2BF Antibody EP858Y
Also for HIST2H2BF (NM_001024599)
|A synthetic acetylated peptide corresponding to residues surrounding Lys12 of Histone H2B was used as an immunogen. The antibody only detects Histone H2B acetylated on Lysine 12.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:2000; IHC: 1:250 500; ICC: 1:250 500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens histone cluster 2, H2bf (HIST2H2BF), transcript variant 1|
|FLJ35099; FLJ56780; FLJ56787; MGC131639|
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Western blot - Histone H2B antibody [EP858Y]; Anti-Histone H2B (acetyl K12) antibody [EP858Y] at 1/2000 dilution + C6+TSA cell lysate at 10 µg.Predicted band size : 14 kDa.Observed band size : 15 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Histone H2B (acetyl K12) antibody [EP858Y]; Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using anti-Histone H2B ab40883 at a 1:250 dilution.
Immunocytochemistry/ Immunofluorescence - Histone H2B (acetyl K12) antibody [EP858Y]; ab40883 (1:250) staining of HeLa cells by immunofluorescence.
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K12) antibody [EP858Y]; ab40883 (1/500) staining Histone H2B (acetyl K12) in assynchronous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised using 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.