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Home Antibody All anti-HIST2H2BF antibodies

Anti-HIST2H2BF Antibody EP858Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303807
  • Rabbit monoclonal antibody against Histone H2B, Acetyl-(K12)(EP858Y)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC422516) , 20ug
100ul $325 3-7 Days
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WB(1)
IHC(1)
IF(2)
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Also for HIST2H2BF (NM_001024599)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic acetylated peptide corresponding to residues surrounding Lys12 of Histone H2B was used as an immunogen. The antibody only detects Histone H2B acetylated on Lysine 12.
Clone NameEP858Y IsotypeIgG
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:2000; IHC: 1:250 500; ICC: 1:250 500
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens histone cluster 2, H2bf (HIST2H2BF), transcript variant 1
Alternative NameFLJ35099; FLJ56780; FLJ56787; MGC131639
Database LinkNP_001019770
Function
Related Pathway

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WB Image
Western blot - Histone H2B antibody [EP858Y]; Anti-Histone H2B (acetyl K12) antibody [EP858Y] at 1/2000 dilution + C6+TSA cell lysate at 10 µg.Predicted band size : 14 kDa.Observed band size : 15 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Histone H2B (acetyl K12) antibody [EP858Y]; Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using anti-Histone H2B TA303807 at a 1:250 dilution.
IF Image
Immunocytochemistry/ Immunofluorescence - Histone H2B (acetyl K12) antibody [EP858Y]; TA303807 (1:250) staining of HeLa cells by immunofluorescence.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Histone H2B (acetyl K12) antibody [EP858Y]; TA303807 (1/500) staining Histone H2B (acetyl K12) in assynchronous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised using 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

 

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