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Home Antibody All anti-HIST1H3J antibodies

Anti-HIST1H3J PHOSPHO Antibody EPR5930

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA310576
  • Rabbit Monoclonal antibody against Histone H3, pT11 (HIST1H3J) (Phospho-specific)
100ul 325 3-7 Days
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WB(1)
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Also for HIST1H3J (NM_003535)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenA phospho specific peptide corresponding to residues surrounding Threonine 11 of human Histone H3 was used as an immunogen. This antibody only detects Histone H3 phosphorylated at Theronine 11.
Clone NameEPR5930 IsotypeTissue culture supernatant
Species ReactivityRat, Human (Does not react with: Mouse) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:1000 - 1:10000
BufferDoes not react with Mouse. Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
Purification pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Note Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

Reference Data

Target NameHomo sapiens histone cluster 1, H3j (HIST1H3J)
Alternative NameH3/j; H3FJ
Database LinkNP_003526
FunctionChanges in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H3 is primarily acetylated at lysines 9, 14, 18, and 23 (3,4). Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (3,4). Phosphorylation at Threonine 11 occurs preferentially at the centromere from prophase to anaphase (5).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-Histone H3 (phospho T11) antibody [EPR5930]; All lanes : Anti-Histone H3 (phospho T11) antibody [EPR5930] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HeLa cell lysate treated with FBS and Calyculin A.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit IgG at 1/2000 dilution.Predicted band size : 15 kDa.Observed band size : 17 kDa .

 

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