Home Antibody All anti-HIST1H3F antibodies
Anti-HIST1H3F PHOSPHO Antibody EP1702Y
Also for HIST1H3F (NM_021018)
|A phospho specific peptide corresponding to residues surrounding Threonine 3 of human Histone H3 was used as an immunogen. This antibody detects Histone H3 phosphorylated at Theronine 3.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||FC: 1:100; WB: 1:500000 - 1:1e+006; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens histone cluster 1, H3f (HIST1H3F)|
|Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. |
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Western blot - Histone H3 (phospho T3) antibody; All lanes : Anti-Histone H3 (phospho T3) antibody at 1/500000 dilution.Lane 1 : HeLa cell lysates.Lane 2 : HeLa cell lysates treated with FBS + Calyculin A.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 16 kDa.Observed band size : 14 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H3 (phospho T3) antibody; ab78351 at 1/100 dilution staining Histone H3 in human breast carcinoma tissue, using a HRP/AP polymerized secondary antibody.
Flow Cytometry-Anti-Histone H3 (phospho T3) antibody(ab78351); Overlay histogram showing HeLa cells stained with ab78351 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.