A synthetic acetylated peptide corresponding to residues surrounding Lys 56 of Histone H3 was used as immunogen. The antibody only detects Histone H3 acetylated on Lysine 56.
Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Related Pathway
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Western blot analysis on C6 cell lysate using anti-Histone H3 Acetyl-K56 RabMAb<br> (TA303820), dilution 1:2000. Cells were either (A) untreated (B) treated with TSA.
