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Anti-HIPK2 Antibody EPR3819
Also for HIPK2 (NM_001113239)
|A synthetic peptide corresponding to residues in human HIPK2 was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:2000 - 1:10000; IP: 1:20; ICC: 1:100 - 1:250; FC: 1:10
|Does not react with Rat. Is unsuitable for IHC-P.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for IHC-P.
|Homo sapiens homeodomain interacting protein kinase 2 (HIPK2), transcript variant 2|
|Homeodomain-interacting protein kinase 2 (HIPK2) is a conserved serine/threonine nuclear kinase that interacts with homeodomain transcription factors and acts as a corepressor of several transcription factors, including SMAD1 and POU4F1/Brn3a and probably NK homeodomain transcription factors (1). In most cell types, HIPK2 activation is associated with the induction of growth suppression and apoptosis, which is mediated through both p53-dependent and p53-independent HIPK2 signaling routes. HIPK2 was found to be a DNA damage-responsive kinase that gets activated upon UV, ionizing irradiation and chemotherapeutic drug treatment (2). In response to severe DNA damage, HIPK2 forms a complex with p53 and phosphorylates p53 at Ser46, which in turn activates apoptotic p53-target genes such as PUMA, Bax, and Noxa (3). Upon DNA damage in the absence of p53, HIPK2 phosphorylates anti-apoptotic corepressor C-terminal binding protein (CtBP) at Ser422, leading to proteasomal degradation of CtBP (4). HIPK2 activity can be restrained by relocalizing into the cytoplasm mediated by HMGA1 (5) or the E3 ubiquitin ligases Siah-1 and WSB-1-mediated proteasome-dependent degradation (6,7).|
Wnt Signaling Pathway
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Western blot - HIPK2 antibody [EPR3819]; All lanes : Anti-HIPK2 antibody [EPR3819] at 1/2000 dilution.Lane 1 : HT-1080 cell lysate.Lane 2 : HepG2 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 131 kDa.Observed band size : 130 kDa .Additional bands at : 100 kDa (possible isoform).