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Home Antibody All anti-HIF1A antibodies

Anti-HIF1A Antibody H1alpha67

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SKU Description Amount Price Availability*  
TA309691
  • Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419880) , 20ug Explanation
0.1ml 325 3 Weeks
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WB(2)
IP(1)
IHC(1)

OriGene Data

ImmunogenFusion protein containing amino acids 432-528 of human HIF-1 alpha
Clone NameH1alpha67 IsotypeIgG2b
Species ReactivityBovine, Ferret, Human, Mouse, Porcine, Primate, Rat, Sheep ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IP Suggested DilutionsWB, IF, IHC
BufferTris-glycine, 150mM NaCl pH 7.5 and 0.05% Sodium Azide
Purification Protein A purified

Reference Data

Target NameHomo sapiens hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 1
Alternative NamebHLHe78; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8
Database LinkNP_001521
Entrez Gene 3091 Human
Entrez Gene 15251 Mouse
Entrez Gene 29560 Rat
FunctionHypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.
Related PathwayTranscription Factors mTOR signaling pathwayPathways in cancerRenal cell carcinoma

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western Blot: HIF-1 alpha Antibody (H1alpha67) - On day 1, MEF cells (+/+,-/-), were grown on 15 cm dish (2x10 to the 6th cells). On day 2, cells were exposed to hypoxia for 4hrs. Cells were washed with ice cold PBS twice and whole cell protein was extracted with RIPA buffer fortified with protease. Upon quantification, 100ug of protein was fractionated on 7% polyacralymide gel. -Gel was transferred overnight onto nitrocellulose membrane. The membrane was probed with HIF-1 alpha monoclonal antibody at a 1:500 dilution . The secondary antibody was conjugated with HRP and was used at a 1:2500 dilution. Photo courtesy of Dr. Gregg Semenza, Johns Hopkins University.
WB Image
Western Blot: HIF-1 alpha Antibody (H1alpha67) - Detection of HIF-1 alpha in cobalt chloride treated and untreated COS-7 nuclear extracts.
IHC Image
Immunohistochemistry: HIF-1 alpha Antibody (H1alpha67) - Staining of HIF-1 alpha in human kidney.
IP Image
Immunoprecipitation: HIF-1 alpha Antibody (H1alpha67) - Heavy and light chains are also detected.

 

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