HIF-1 alpha (HIF1A) Mouse Monoclonal Antibody [Clone ID: H1alpha67]

CAT#: TA309691

Mouse Monoclonal HIF-1 alpha Antibody (H1alpha67)


USD 511.00

2 Weeks*

Size
    • 100 ul

Product Images

Frequently bought together (2)
beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control
    • 30 ul

USD 200.00


Transient overexpression lysate of hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 2
    • 100 ug

USD 665.00

Other products for "HIF-1 alpha"

Specifications

Product Data
Clone Name H1alpha67
Applications ChIP, ELISA, FC, ICC/IF, IHC, Immunoblotting, IP, WB
Recommended Dilution Chromatin Immunoprecipitation: 1:10 - 1:500, Western Blot: 1:500 - 1:1000, Flow Cytometry: 1:10 - 1:1000, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry: 1:100 - 1:300, Immunohistochemistry-Paraffin: 1:100 - 1:300, Immunoprecipitation: 1:10, Gel Super Shift Assays: 1:1 - 1:100, Knockout Validated, Chromatin Immunoprecipitation (ChIP): 1:10-1:500, ELISA: 1:100 - 1:2000, Immunoblotting, Knockdown Validated
Reactivities Bovine, Ferret, Human, Mouse, Porcine, Primate, Rat, Sheep
Host Mouse
Isotype IgG2b
Clonality Monoclonal
Immunogen Fusion protein containing amino acids 432-528 of human HIF-1 alpha
Formulation Tris-glycine, 150mM NaCl pH 7.5 and 0.05% Sodium Azide
Purification Protein A purified
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Gene Name hypoxia inducible factor 1 alpha subunit
Background Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.
Synonyms bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8
Reference Data
Protein Families Transcription Factors
Protein Pathways mTOR signaling pathway, Pathways in cancer, Renal cell carcinoma

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.