OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-HICE1 antibodies

Anti-HICE1 Antibody

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319225
  • Rabbit polyclonal anti-HICE1 antibody
  • FREE positive control: HEK293T cell transient overexpression lysate (LC409542) , 20ug
100ul 325 3-7 Days
Add to Shopping Cart
WB(1)

OriGene Data

ImmunogenAnti-HICE1 was prepared from whole rabbit serum produced by repeated immunizations with a recombinant full length Hice1 protein.
Clone Name IsotypeIgG
Species ReactivityHuman Concentration38 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:250,000, WB: 1:10,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Hice1 contributes to the mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex. Normal bipolar spindle formation is critical for accurate chromosome segregation and proper mitotic progression. Failure in this event leads to spindle checkpoint activation and chromosome missegregation that ultimately leads to aneuploidy. Hice1 binds to microtubules directly, and promotes spindle integrity and chromosome stability. Hice1 has also shown to play an important role in targeting the ?TuRC complex to the mitotic spindle, a step that appears to be required for spindle-mediated microtubule generation and normal chromosome segregation. The HAUS augmin-like complex's interaction with microtubules is strong during mitosis, while it is weak or absent during interphase. During interphase, it is primarily cytoplasmic, associating with centrosomes and with the mitotic spindles, preferentially at the spindle pole vicinity. During anaphase and telophase, it additionally associates with the spindle midzone and midbody, respectively. Further characterization of the function of Hice1 will likely be important for better understanding the mechanism of normal mitotic progression and high fidelity chromosome segregation.

Reference Data

Target NameHomo sapiens HAUS augmin-like complex, subunit 8 (HAUS8), transcript variant 2
Alternative NameDGT4; HICE1; NY-SAR-48
Database LinkNP_001011699
Function
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Anti-HICE1 in Western Blot using Immunochemicals' Anti-HICE1 Antibody shows detection of a 45 kDa band corresponding to endogenous HICE1 in lysates of S phase HeLa cells silenced for either control Luciferase or HICE1. In right lane (HICE1i): lysates from sh-HICE1 RNAi-treated lentivirus-infected cells. In left lane (GLi): lysates from sh-Luciferase lentivirus-infected cells as control. Anti-HICE1 Antibody was used at 1:10,000. Molecular weight estimation was made by comparison by prestained MW markers. ECL was used for detection. Personal communication, Kyung S. Lee, NCI, Bethesda, MD.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title