This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box
Delta-Notch Signaling Pathway
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HEK293T cells were transfected with pCMV6-ENTRY control (Lane 1) or pCMV6-ENTRY HES1 (Lane 2) plasmids for 48 hrs. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1-HRP.
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