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Anti-HDAC6 TRUEMAB Antibody clone 5E6
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for HDAC6 (NM_006044)
|Full length human recombinant protein of human HDAC6 (NP_006035) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens histone deacetylase 6 (HDAC6)|
|CPBHM; HD6; JM21|
|Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC6 (RC209649, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC6.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HDAC6 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-HDAC6 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502082)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HDAC6 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502082)
Anti-HDAC6 mouse monoclonal antibody (TA502082) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HDAC6(RC209649).
HEK293T cells transfected with either RC209649 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-HDAC6 antibody(TA502082), and then analyzed by flow cytometry.