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Home Antibody All anti-HDAC2 antibodies

Anti-HDAC2 PHOSPHO Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA323155
  • Anti-HDAC2 (Phospho-Ser394) Rabbit Polyclonal Antibody
100ul 325 2-3 Weeks
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WB(1)
IHC(1)
IF(1)

OriGene Data

ImmunogenPeptide sequence around phosphorylation site of serine 394 (E-D-S(p)-G-D) derived from Human HDAC2.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:500-1000, IHC: 1:50-100, IF: 1:100-200
Predicted MW Explanation 55kDa
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens histone deacetylase 2 (HDAC2), transcript variant 1
Alternative NameHD2; RPD3; YAF1
Database LinkNP_001518
Entrez Gene 3066 Human
Entrez Gene 15182 Mouse
Entrez Gene 84577 Rat
FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes
Related PathwayStem cell - PluripotencyTranscription FactorsDruggable Genome Cell cycleNotch signaling pathwayHuntington's diseasePathways in cancerChronic myeloid leukemia

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 55 kDa . Positive control: 293 cells treated with EGF lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: 293 cells untreated with EGF lysate Lane2: 293 cells treated with EGF lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
IHC Image
Predicted cell location: Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using HDAC2 (Phospho-Ser394) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)
IF Image
Predicted cell location: Nuclear. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using HDAC2 (Phospho-Ser394) antibody at dilution 1/100. (Original magnification: ×200)

 

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