Anti-H6PD Antibody, clone 2A7

Also for H6PD (NM_004285)

Gene Name	Homo sapiens hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) (H6PD)
Synonyms:	CORTRD1; G6PDH; GDH
Immunogen	Full length human recombinant protein of human H6PD (NP_004276) produced in HEK293T cell.
Buffer	PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 2A7	Isotype	IgG1
Species Reactivity	Human, Monkey	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, IHC, IF, FC	Suggested Dilutions	WB 1:250~500, IHC 1:50, IF 1:100, FLOW 1:100
Background	There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form, encoded by this gene, is autosomally linked. This H form shows activity with other hexose-6-phosphates, especially galactose-6-phosphate, whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues, but H form is not found in red cells.
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY H6PD (RC209890, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-H6PD .

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-H6PD monoclonal antibody.

IHC Image

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-H6PD mouse monoclonal antibody. (TA501257, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-H6PD mouse monoclonal antibody. (TA501257, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-H6PD mouse monoclonal antibody. (TA501257, Dilution 1:50)

IHC Image

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-H6PD mouse monoclonal antibody. (TA501257, Dilution 1:50)

IF Image

Anti-H6PD mouse monoclonal antibody (TA501257) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY H6PD (RC209890).

FC Image

HEK293T cells transfected with either RC209890 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-H6PD antibody(TA501257), and then analyzed by flow cytometry.

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-H6PD antibody(TA501257),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

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Anti-H6PD TrueMAB Antibody clone 2A7

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