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Home Antibody All anti-H3F3B antibodies

Anti-H3F3B Antibody, Loading Control

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SKU Description Amount Price Availability*  
TA308895
  • Rabbit polyclonal anti-H3F3B(Histone H3)antibody, Loading control
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401642) , 20ug Explanation
100ul $325 3-7 Days
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WB(3)
IHC(3)
IF(2)
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Also for H3F3B (NM_005324)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenRecombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 86 of Histone H3.3B
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.44mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:500 - 1:3000, IHC 1:100 - 1:1000, IF 1:100 - 1:1000
Predicted MW Explanation 15kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Note Seq homology of immunogen across species: Xenopus laevis (100%), Xenopus Tropicalis (100%), Chicken (100%), Bovine (100%)

Reference Data

Target NameHomo sapiens H3 histone, family 3B (H3.3B) (H3F3B)
Alternative NameH3.3B
Database LinkNP_005315
Entrez Gene 3021 Human
Entrez Gene 15081 Mouse
FunctionHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is poyadenylated, unlike most histone genes. The protein encoded is a member of the histone H3 family. [provided by RefSeq]
Related Pathway Systemic lupus erythematosus

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Sample (50 ug of whole cell lysate). A: mouse brain. 15% SDS PAGE. TA308895 diluted at 1:1000.
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Histone H3.3B antibody detects H3F3B protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 15 % SDS-PAGE. Histone H3.3B antibody (TA308895) dilution: 1:1000
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Sample (30 ug of whole cell lysate). A: Hela. 15% SDS PAGE. TA308895 diluted at 1:1000.
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Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Histone H3.3B antibody (TA308895) dilution: 1:500.
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Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Histone H3.3B antibody (TA308895) dilution: 1:500.
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Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using Histone H3.3B(TA308895) antibody at 1:500 dilution.
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Histone H3.3B antibody detects Histone H3.3B protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3.3B protein stained by Histone H3.3B antibody (TA308895) diluted at 1:50
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Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Histone H3.3B(TA308895) antibody at 1:200 dilution.
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