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Home Antibody All anti-GYS1 antibodies

Anti-GYS1 Antibody EP852Y

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Specifications Citations (1) Related Products Product Documents
SKU Description Amount Price Availability*  
TA300820 Rabbit Monoclonal Antibody against GYS1 (Clone EP852Y) 100ul $325 In Stock
LC400769 GYS1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(2)
IHC(1)
IF(1)

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Ser641 of human Glycogen Synthase was used as immunogen. The antibody only detects Glycogen Synthase phosphorylated on Serine 641.
Clone NameEP852Y IsotypeIgG
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:10000; IHC-P: 1:100 - 1:250; ICC: 1:50 - 1:100; IP: 1:60
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens glycogen synthase 1 (muscle) (GYS1), transcript variant 1
Alternative NameGSY; GYS
Database LinkNP_002094
Entrez Gene 2997 Human
Entrez Gene 14936 Mouse
FunctionGlycogen synthase is an enzyme of the transferase class that catalyses the reaction of UDP-glucose and (1,4-?-D-glucosyl)n to yield UDP and (1,4-?-D-glucosyl)n+1 (1-2). This tetrameric enzyme is the rate-limiting step for glycogen synthesis (3). Glycogen concentrations are regulated by the complementary activities of glycogen phosphorylase and glycogen synthase. Glycogen synthase activity is regulated by phosphorylation of serine residues and by insulin stimulation (4). Glycogen synthase is known to have two forms; the unphosphorylated and most active form, synthase-a, and the phosphorylated glucose-6-phosphate-dependent form, synthase-b.
Related Pathway Starch and sucrose metabolismInsulin signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Glycogen synthase 1 (phospho S641) antibody [EP852Y]; All lanes : Anti-Glycogen synthase 1 (phospho S641) antibody [EP852Y] at 1/10000 dilution.Lane 1 : Mouse muscle lysate, untreated.Lane 2 : Mouse muscle lysate, treated with AP.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled Goat anti-Rabbit at 1/2000 dilution.Predicted band size : 85 kDa.Observed band size : 85 kDa.
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Figure from citation: Western Blot of GYS1 protein level by using anti-GYS1 antibody in mouse muscle extracts obtained from Gbe1-/-. Gbe1+/- and Gbe1+/+ embryos. View Citation
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Glycogen synthase 1 (phospho S641) antibody [EP852Y]; Immunohistochemical analysis of Human liver tissue, using 1/100 TA300820
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ICC/IF image of TA300820 stained A431 cells. The cells were incubated with the antibody overnight at 4.. The secondary antibody (green) was , DyLight 488 goat anti-rabbit IgG (H+L) used at 1:250. for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
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