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Home Antibody All anti-GYS1 antibodies

Anti-GYS1 Antibody EP816Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300659
  • Rabbit Monoclonal Antibody against GYS1 (Clone EP816Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400769) , 20ug
100ul 325 In Stock
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WB(1)
IHC(1)
IF(1)
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Also for GYS1 (NM_002103)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of the human glycogen synthase was used as an immunogen.
Clone NameEP816Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB: 1:20,000; IHC: 1:250 500; ICC: 1: 100 250; IP: 1:50
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens glycogen synthase 1 (muscle) (GYS1), transcript variant 1
Alternative NameGSY; GYS
Database LinkNP_002094
FunctionGlycogen synthase is an enzyme of the transferase class that catalyses the reaction of UDP-glucose and (1,4-a-D-glucosyl)n to yield UDP and (1,4-a-D-glucosyl)n+1 (1-2). This tetrameric enzyme is the rate-limiting step for glycogen synthesis (3). Glycogen concentrations are regulated by the complementary activities of glycogen phosphorylase and glycogen synthase. Glycogen synthase activity is regulated by phosphorylation of serine residues and by insulin stimulation (4). Glycogen synthase is known to have two forms; the unphosphorylated and most active form, synthase-a, and the phosphorylated glucose-6-phosphate-dependent form, synthase-b.
Related Pathway

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WB Image
Western blot - Glycogen synthase 1 antibody [EP816Y]; Anti-Glycogen synthase 1 antibody [EP816Y] at 1/20000 dilution + HeLa cell lysate at 10 µg.Predicted band size : 81 kDa.Observed band size : 80 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Glycogen synthase 1 antibody [EP816Y]; Immunohistochemical staining of paraffin-embedded human muscle using anti-Glycogen Synthase 1 TA300659 diluted 1:250.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Glycogen synthase 1 antibody [EP816Y]; ICC/IF image of TA300659 stained A431 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was , DyLight? 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

 

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