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Anti-GYS1 Antibody EP817Y
Also for GYS1 (NM_002103)
|A synthetic peptide corresponding to residues in the C-terminus of the human glycogen synthase was used as immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
||IHC-P: Use at an assay dependent concentration; WB: 1:10000 - 1:50000; ICC/IF: 1:250 - 1:500; FC: 1:50 - 1:1000; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified (Protein A or G Sepharose)
|Homo sapiens glycogen synthase 1 (muscle) (GYS1), transcript variant 1|
|Glycogen synthase is an enzyme of the transferase class that catalyses the reaction of UDP-glucose and (1,4-a-D-glucosyl)n to yield UDP and (1,4-a-D-glucosyl)n+1 (1-2). This tetrameric enzyme is the rate-limiting step for glycogen synthesis (3). Glycogen concentrations are regulated by the complementary activities of glycogen phosphorylase and glycogen synthase. Glycogen synthase activity is regulated by phosphorylation of serine residues and by insulin stimulation (4). Glycogen synthase is known to have two forms; the unphosphorylated and most active form, synthase-a, and the phosphorylated glucose-6-phosphate-dependent form, synthase-b.|
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Western blot - Glycogen synthase 1 antibody [EP817Y]; Anti-Glycogen synthase 1 antibody [EP817Y] at 1/50000 dilution + HeLa cell lysate at 10 µg.Predicted band size : 81 kDa.Observed band size : 85 kDa .
Immunocytochemistry/ Immunofluorescence - Glycogen synthase 1 antibody [EP817Y]; ab40810 at a 1:250 dilution staining Hela cells using anti-Glycogen Synthase 1 RabMAb.
Flow Cytometry - Anti-Glycogen synthase 1 antibody [EP817Y]; Overlay histogram showing HEK293 cells stained with ab40810 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.