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Home Antibody All anti-GYS1 antibodies

Anti-GYS1 Antibody EP817Y

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA300640 Rabbit Monoclonal Antibody against GYS1 (Clone EP817Y) 100ul $325 In Stock
LC400769 GYS1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)
IF(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the C-terminus of the human glycogen synthase was used as immunogen.
Clone NameEP817Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF, FC Suggested DilutionsIHC-P: Use at an assay dependent concentration; WB: 1:10000 - 1:50000; ICC/IF: 1:250 - 1:500; FC: 1:50 - 1:1000; IP: 1:50
Predicted MW Explanation 81 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Protein A purified

Reference Data

Target NameHomo sapiens glycogen synthase 1 (muscle) (GYS1), transcript variant 1
Alternative NameGSY; GYS
Database LinkNP_002094
Entrez Gene 2997 Human
Entrez Gene 14936 Mouse
Entrez Gene 690987 Rat
FunctionGlycogen synthase is an enzyme of the transferase class that catalyses the reaction of UDP-glucose and (1,4-a-D-glucosyl)n to yield UDP and (1,4-a-D-glucosyl)n+1 (1-2). This tetrameric enzyme is the rate-limiting step for glycogen synthesis (3). Glycogen concentrations are regulated by the complementary activities of glycogen phosphorylase and glycogen synthase. Glycogen synthase activity is regulated by phosphorylation of serine residues and by insulin stimulation (4). Glycogen synthase is known to have two forms; the unphosphorylated and most active form, synthase-a, and the phosphorylated glucose-6-phosphate-dependent form, synthase-b.
Related Pathway Starch and sucrose metabolismInsulin signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Glycogen synthase 1 antibody [EP817Y]; Anti-Glycogen synthase 1 antibody [EP817Y] at 1/50000 dilution + HeLa cell lysate at 10 ug.Predicted band size : 81 kDa.Observed band size : 85 kDa .
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Immunocytochemistry/ Immunofluorescence - Glycogen synthase 1 antibody [EP817Y]; TA300640 at a 1:250 dilution staining Hela cells using anti-Glycogen Synthase 1 RabMAb.
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Flow Cytometry - Anti-Glycogen synthase 1 antibody; Overlay histogram showing HEK293 cells stained with TA300640 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) used under the same conditions. Unlabelled sample (blue line) was also used as a control. This antibody gave a positive signal in HEK293 cells under the same conditions.
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