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Anti-GYS1 Antibody EP817Y
Also for GYS1 (NM_002103)
|A synthetic peptide corresponding to residues in the C-terminus of the human glycogen synthase was used as immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IF, FC
||IHC-P: Use at an assay dependent concentration; WB: 1:10000 - 1:50000; ICC/IF: 1:250 - 1:500; FC: 1:50 - 1:1000; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified
|Homo sapiens glycogen synthase 1 (muscle) (GYS1), transcript variant 1|
Entrez Gene 2997 Human
Entrez Gene 14936 Mouse
Entrez Gene 690987 Rat
|Glycogen synthase is an enzyme of the transferase class that catalyses the reaction of UDP-glucose and (1,4-a-D-glucosyl)n to yield UDP and (1,4-a-D-glucosyl)n+1 (1-2). This tetrameric enzyme is the rate-limiting step for glycogen synthesis (3). Glycogen concentrations are regulated by the complementary activities of glycogen phosphorylase and glycogen synthase. Glycogen synthase activity is regulated by phosphorylation of serine residues and by insulin stimulation (4). Glycogen synthase is known to have two forms; the unphosphorylated and most active form, synthase-a, and the phosphorylated glucose-6-phosphate-dependent form, synthase-b.|
| Starch and sucrose metabolismInsulin signaling pathway|
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Western blot - Glycogen synthase 1 antibody [EP817Y]; Anti-Glycogen synthase 1 antibody [EP817Y] at 1/50000 dilution + HeLa cell lysate at 10 ug.Predicted band size : 81 kDa.Observed band size : 85 kDa .
Immunocytochemistry/ Immunofluorescence - Glycogen synthase 1 antibody [EP817Y]; TA300640 at a 1:250 dilution staining Hela cells using anti-Glycogen Synthase 1 RabMAb.
Flow Cytometry - Anti-Glycogen synthase 1 antibody; Overlay histogram showing HEK293 cells stained with TA300640 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) used under the same conditions. Unlabelled sample (blue line) was also used as a control. This antibody gave a positive signal in HEK293 cells under the same conditions.