OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-GYS1 antibodies

Anti-GYS1 Antibody EP817Y

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300640
  • Rabbit Monoclonal Antibody against GYS1 (Clone EP817Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400769) , 20ug Explanation
100ul 325 In Stock
Add to Shopping Cart
WB(1)
IF(1)
FC(1)
spacer
Also for GYS1 (NM_002103)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the C-terminus of the human glycogen synthase was used as immunogen.
Clone NameEP817Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF, FC Suggested DilutionsIHC-P: Use at an assay dependent concentration; WB: 1:10000 - 1:50000; ICC/IF: 1:250 - 1:500; FC: 1:50 - 1:1000; IP: 1:50
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Protein A purified

Reference Data

Target NameHomo sapiens glycogen synthase 1 (muscle) (GYS1), transcript variant 1
Alternative NameGSY; GYS
Database LinkNP_002094
Entrez Gene 2997 Human
Entrez Gene 14936 Mouse
Entrez Gene 690987 Rat
FunctionGlycogen synthase is an enzyme of the transferase class that catalyses the reaction of UDP-glucose and (1,4-a-D-glucosyl)n to yield UDP and (1,4-a-D-glucosyl)n+1 (1-2). This tetrameric enzyme is the rate-limiting step for glycogen synthesis (3). Glycogen concentrations are regulated by the complementary activities of glycogen phosphorylase and glycogen synthase. Glycogen synthase activity is regulated by phosphorylation of serine residues and by insulin stimulation (4). Glycogen synthase is known to have two forms; the unphosphorylated and most active form, synthase-a, and the phosphorylated glucose-6-phosphate-dependent form, synthase-b.
Related Pathway Starch and sucrose metabolismInsulin signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Glycogen synthase 1 antibody [EP817Y]; Anti-Glycogen synthase 1 antibody [EP817Y] at 1/50000 dilution + HeLa cell lysate at 10 ug.Predicted band size : 81 kDa.Observed band size : 85 kDa .
IF Image
Immunocytochemistry/ Immunofluorescence - Glycogen synthase 1 antibody [EP817Y]; TA300640 at a 1:250 dilution staining Hela cells using anti-Glycogen Synthase 1 RabMAb.
FC Image
Flow Cytometry - Anti-Glycogen synthase 1 antibody [EP817Y]; Overlay histogram showing HEK293 cells stained with TA300640 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title