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Anti-GSK3A PHOSPHO Antibody EPR933Y
Also for GSK3A (NM_019884)
|A phospho specific peptide corresponding to residues surrounding tyrosine 279 in human GSK3a and tyrosine 216 in human GSKb was used as an immunogen. This antibody detects both GSK? phosphorylated on tyrosine 279 and GSK3? phosphorylyated on tyrosine 216.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
||WB: 1:500~2000; IHC: 1: 100; ICC: 1:250; IP: 1:40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Homo sapiens glycogen synthase kinase 3 alpha (GSK3A)|
|Glycogen synthases kinase 3 alpha (GSK3-?) is a multifunctional Serine/Threonine protein kinase, one of two isoforms of GSK3 (1). Originally identified as a regulator of glycogen synthase, it has been shown to be involved in the hormonal control of several regulatory factors and transcription factors. GSK3-? activity is down regulated by phosphorylation on Serine 21 by PKB, or activated by phosphorylation on Tyrosine 279 (2). Insulin inactivates GSK3-? by rapid phosphorylation which leads to activation of Glycogen Synthase in the cells (3). GSK3-? activitiy has been linked to a pathogenic mechanism in Alzheimer's disease (4). GSK3 beta) plays a key role in insulin and Wnt signaling, phosphorylating downstream targets by default, and becoming inhibited following the extracellular signaling event (5). |
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Western blot - GSK3 (alpha + beta) (phospho Y279 + Y216) antibody [EPR933Y]; All lanes : Anti-GSK3 (alpha + beta) (phospho Y279 + Y216) antibody [EPR933Y] at 1/2000 dilution.Lane 1 : 293 cell lysate, cells untreated.Lane 2 : 293 cell lysate, cells treated with AP.Lysates/proteins at 10 µg per lane.Secondary.HRP conjugated Goat anti-rabbit at 1/2000 dilution.Predicted band size : 51 , 47 kDa.Observed band size : 47-52 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GSK3 (alpha + beta) (phospho Y279 + Y216) antibody [EPR933Y]; Human brain tissue stained with TA301166 at 1/100 dilution.