Home Antibody All anti-GRN antibodies
Anti-GRN GAT Antibody Polyclonal
Generated by Genomic Immunization
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|DNA immunization. This antibody is specific for the Middle Region of the target protein.|
|Human , Dog , Monkey , Mouse
|WB, ELISA, IHC, IF
|| WB 1:5000~20000,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens granulin (GRN)|
|CLN11; GEP; GP88; PCDGF; PEPI; PGRN|
Entrez Gene 2896 Human
Entrez Gene 14824 Mouse
Entrez Gene 714851 Monkey
Entrez Gene 480501 Dog
|Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.|
|Secreted ProteinDruggable Genome |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (RC202139, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GRN polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
ELISA: Granulin Antibody - Affinity Purified
Immunohistochemical staining of human epididymis shows strong cytoplasmic positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of human cell line U-2 OS shows no positivity.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.