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Anti-GHR Antibody EPR5291(2)
|A synthetic peptide corresponding to residues in the intracellular domain in human Growth Hormone Receptor was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, ASSAY, IF, FC
||WB: 1:1000 - 1:10000; FC: 1:100 - 1:500; ICC/IF: 1:100 - 1:250
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for IHC-P or IP.
|Homo sapiens growth hormone receptor (GHR), transcript variant 1|
Entrez Gene 2690 Human
Entrez Gene 14600 Mouse
Entrez Gene 25235 Rat
|Growth Hormone Receptor is a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in Growth Hormone Receptor have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein (1).|
|TransmembraneDruggable Genome Cytokine-cytokine receptor interactionNeuroactive ligand-receptor interactionJak-STAT signaling pathway|
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Western blot - Anti-Growth hormone receptor antibody [EPR5291(2)]; All lanes : Anti-Growth hormone receptor antibody [EPR5291(2)] at 1/1000 dilution.Lane 1 : MCF-7 lysate.Lane 2 : BxPC-3 lysate .Lane 3 : Fetal muscle lysate .Lysates/proteins at 10 ug per lane.Secondary.HRP labelled Goat anti-rabbit at 1/2000 dilution.Predicted band size : 72 kDa.Observed band size : 140 kDa .
Other-Anti-Growth hormone receptor antibody [EPR5291(2)](TA310531); Equilibrium disassociation constant (KD)..
Immunocytochemistry/ Immunofluorescence - Anti-Growth hormone receptor antibody [EPR5291(2)]; TA310531 staining Growth hormone receptor in a Human Glioblastoma cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde,permeabilized with TBS Tween 0.5% and blocked for 60 minutes at 25Â°C. Samples were incubated with primary antibody (1/100) for 20 hour at 4Â°C. An Alexa Fluor?594-conjugated Goat anti-rabbit IgG polyclonal(1/200) was used as the secondary antibody.
Flow Cytometry - Anti-Growth hormone receptor antibody [EPR5291(2)]; Flow cytometrical analysis of permeabilized BxPC-3 cells labelling Growth hormone receptor (red) with TA310531 at 1/100 and a rabbit IgG in negative (green).