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Anti-GFAP Antibody EPR1034Y
Also for GFAP (NM_002055)
|A synthetic peptide corresponding to residues in the N-term of human GFAP was used as immunogen.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
||WB: 1:50000 - 1:100000; IP: 1:40; IHC-P: 1:250 - 1:500; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt.
|Homo sapiens glial fibrillary acidic protein (GFAP), transcript variant 1|
|Glial fibrillary acidic protein (GFAP) belongs to the class III of the intermediate filament (IF) proteins highly specific to astrocytes which function as nerve cells in the brain and in the central nervous system (CNS) (1-2). Two other class III IF proteins, nestin and vimentin, dominate in immature astrocytes, while GFAP is most abundant in mature astrocytes (3). GFAP is used as a marker to distinguish astrocytes form other glial cells during development. Mutations in GFAP cause Alexander disease, a rare disorder of the CNS. Most frequent mutations are located at Arginine 79 or 239 (4). |
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Western blot - GFAP antibody [EPR1034Y]; All lanes : Anti-GFAP antibody [EPR1034Y] at 1/5000 dilution.Lane 1 : Human brain lysate.Lane 2 : Rat brain lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution.Predicted band size : 50 kDa.Observed band size : 50 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFAP antibody [EPR1034Y]; Immunohistochemistry on formalin-fixed paraffin-embedded mouse brain using TA301159.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GFAP antibody [EPR1034Y]; Immunohistochemical analysis of paraffin-embedded human brain (left) and human glioma (right) using TA301159