Anti-GBA3 Antibody, clone 5G3

Also for GBA3 (NM_020973)

Gene Name	Homo sapiens glucosidase, beta, acid 3 (cytosolic) (GBA3), transcript variant 1
Synonyms:	CBGL1; GLUC; KLrP
Immunogen	Human recombinant protein fragment corresponding to amino acids 1-150 and 370-469 of human GBA3 (NP_066024) produced in E.coli.
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Name	clone 5G3	Isotype	IgG2b
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *	WB, FC	Suggested Dilutions	WB 1:2000, FLOW 1:100
Background	GBA3, or cytosolic beta-glucosidase (EC 3.2.1.21), is a predominantly liver enzyme that efficiently hydrolyzes beta-D-glucoside and beta-D-galactoside, but not any known physiologic beta-glycoside, suggesting that it may be involved in detoxification of plant glycosides (de Graaf et al., 2001 [PubMed 11389701]). GBA3 also has significant neutral glycosylceramidase activity (EC 3.2.1.62), suggesting that it may be involved in a nonlysosomal catabolic pathway of glucosylceramide metabolism (Hayashi et al., 2007 [PubMed 17595169]).[supplied by OMIM].
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBA3 (RC211035, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBA3.

FC Image

Flow cytometric Analysis of Hela cells, using anti-GBA3 antibody(TA502605),(Red), compared to a nonspecific negative control antibody,(Blue).

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-GBA3 antibody(TA502605),(Red), compared to a nonspecific negative control antibody,(Blue).

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Anti-GBA3 TrueMAB Antibody clone 5G3

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