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Home Antibody All anti-GAD2 antibodies

Anti-GAD2 Antibody


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA308367 Rabbit Polyclonal antibody to GAD65 (glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)) 100ul $325 In Stock
LC427399 GAD2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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Also for GAD2 (NM_001134366)
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OriGene Data

ImmunogenRecombinant fragment corresponding to a region within amino acids 353 and 585 of GAD65 (Uniprot ID#Q05329)
Clone Name IsotypeIgG
Species ReactivityHuman, Mouse (Predicted: Rhesus Monkey) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:1000-1:10000
Predicted MW Explanation 65kDa
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.
Note Seq homology of immunogen across species: Rhesus Monkey (100%)

Reference Data

Target NameHomo sapiens glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) (GAD2), transcript variant 2
Alternative NameGAD65
Database LinkNP_001127838
Entrez Gene 2572 Human
Entrez Gene 14417 Mouse
Entrez Gene 613029 Monkey
FunctionThis gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq]
Related PathwayDruggable Genome Alanine, aspartate and glutamate metabolismbeta-Alanine metabolismTaurine and hypotaurine metabolismButanoate metabolismMetabolic pathwaysType I diabetes mellitusMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Sample (50 ug of whole cell lysate). A: mouse brain. 7.5% SDS PAGE. TA308367 diluted at 1:5000.

WB Image
Sample (50 ug of whole cell lysate). A: rat brain. 7.5% SDS PAGE. TA308367 diluted at 1:1000.

IHC Image
GAD65 antibody detects GAD2 protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. GAD65 antibody (TA308367) dilution: 1:500.

IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GAD65(TA308367) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2500.


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